文献类型： 外文期刊

作者： Qin, Feng ¹ ; Fan, Qiang ¹ ; Yu, Peter K. N. ⁵ ; Almahi, Waleed Abdelbagi ¹ ; Kong, Peizhong ¹ ; Yang, Miaomiao ¹ ; Ca ¹ ;

作者机构： 1.Chinese Acad Sci, Inst Hlth & Med Technol, Hefei Inst Phys Sci, Anhui Prov Key Lab Med Phys & Technol, Hefei, Peoples R China

2.Grad Sch USTC, Sci Isl Branch, Hefei, Peoples R China

3.Chinese Acad Sci, Hefei Canc Hosp, Hefei, Peoples R China

4.Anhui Acad Agr Sci, Inst Sericultural, Hefei, Peoples R China

5.City Univ Hong Kong, Dept Phys, Kowloon Tong, Tat Chee Ave, Hong Kong, Peoples R China

6.City Univ Hong Kong, State Key Lab Marine Pollut, Kowloon Tong, Tat Chee Ave, Hong Kong, Peoples R China

7.Anhui Med Univ, Affiliated Hosp 4, Clin Pathol Ctr, Hefei, Peoples R China

8.Soochow Univ, Collaborat Innovat Ctr Radiat Med, Jiangsu Higher Educ Inst, Suzhou, Peoples R China

9.Soochow Univ, Sch Radiol & Interdisciplinary Sci RAD X, Suzhou, Peoples R China

关键词： Breast cancer; acquired radioresistance; gene expression profiling; energy metabolism

期刊名称：ANNALS OF TRANSLATIONAL MEDICINE （ 影响因子：3.297； ）

ISSN： 2305-5839

年卷期： 2021 年 9 卷 8 期

页码：

收录情况： SCI

摘要： Background: Acquired radioresistant cells exhibit many characteristic changes which may influence cancer progression and further treatment options. The purpose of this study is to investigate the changes of radioresistant human epidermal growth factor receptor 2 (HER2)-positive breast cancer cells on both phenotypic and molecular levels. Methods: We established an acquired radioresistant cell line from its parental NF639 cell line (HER2-positive) by fractionated radiation and assessed changes in cellular morphology, proliferation, migration, anti-apoptosis activity, basal reactive oxygen species (ROS) level and energy metabolism. RNA-sequencing (RNA-seq) was also used to reveal the potential regulating genes and molecular mechanisms associated with the acquired changed phenotypes. Real-time PCR was used to validate the results of RNA-seq. Results: The NF639R cells exhibited increased radioresistance and enhanced activity of proliferation, migration and anti-apoptosis, but decreased basal ROS. Two main energy metabolism pathways, mitochondrial respiration and glycolytic, were also upregulated. Furthermore, 490 differentially expressed genes were identified by RNA-seq. Enrichment analysis based on Gene Ontology and Kyoto Encyclopedia of Genes and Genomes showed many differently expressed genes were significantly enriched in cell morphology, proliferation, migration, anti-apoptosis, antioxidation, tumor stem cells and energy metabolism and the signaling cascades such as the transforming growth factor-beta, Wnt, Hedgehog, vascular endothelial growth factor, retinoic acid-inducible gene I (RIG-I)-like receptor, Toll-like receptor and nucleotide oligomerization domain (NOD)-like receptor were significantly altered in NF639R cells. Conclusions: In clinical radiotherapy, repeat radiotherapy for short-term recurrence of breast cancer may result in enhanced radioresistance and promote malignant progression. Our research provided hints to understand the tumor resistance to radiotherapy de novo and recurrence with a worse prognosis following radiotherapy. Keywords: