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Pear metal transport protein PbMTP8.1 confers manganese tolerance when expressed in yeast and Arabidopsis thaliana

文献类型： 外文期刊

作者： Li, Jiyu 1 ; Zheng, Lu 1 ; Fan, Ye 1 ; Wang, Yuerong 1 ; Ma, Yurou 1 ; Gu, Dongfang 1 ; Lu, Yaping 1 ; Zhang, Shaoling 4 ; C 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Life Sci, Dept Biochem & Mol Biol, Nanjing 210095, Jiangsu, Peoples R China

2.Anhui Acad Agr Sci, Inst Hort, Hefei 230031, Anhui, Peoples R China

3.Key Lab Genet Improvement & Ecophysiol Hort Crops, Hefei 230031, Anhui, Peoples R China

4.Nanjing Agr Univ, Coll Hort, State Key Lab Crop Genet & Germplasm Enhancement, Ctr Pear Engn Technol Res, Nanjing 210095, Jiangsu, Peoples R China

关键词： PbMTP8.1; CDF family; Manganese transporter; Pre-vacuolar compartment

期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY （ 影响因子：6.291； 五年影响因子：6.393 ）

ISSN： 0147-6513

年卷期： 2021 年 208 卷

页码：

收录情况： SCI

摘要： Manganese (Mn) is demonstrated to be essential for plants. Ion homeostasis is maintained in plant cells by specialized transporters. PbMTP8.1, which encodes a putative Mn-CDF transporter in Pyrus bretschneideri Rehd, was expressed mainly in leaves and complemented the Mn hypersensitivity of the Mn-sensitive yeast mutant square pmr1 in previous research conducted by our laboratory. In the present study, we report that the expression of PbMTP8.1 can enhance Mn tolerance and accumulation in Saccharomyces cerevisiae. Subcellular localization analysis of the PbMTP8.1-GFP fusion protein indicated that PbMTP8.1 was targeted to the pre-vacuolar compartment (PVC). In addition, the overexpression of PbMTP8.1 in Arabidopsis thaliana conferred increased resistance to plants under toxic Mn levels, as indicated by increased fresh and dry weights of shoots and roots. Mn accumulation in vacuoles of PbMTP8.1-overexpressing plants was significantly increased when compared with that in wild-type plants under Mn stress. This suggests that a considerable proportion of Mn enters into the vacuoles through a PbMTP8.1-dependent mechanism. Taken together, these results indicate PbMTP8.1 is a Mn-specific transporter that is localized to the PVC, and confers Mn tolerance by sequestering Mn into the vacuole.

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