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Comparative transcriptome and iTRAQ-based proteome analysis in mature leaves of Brassica carinata provides insights into the purple leaf color diversity

文献类型： 外文期刊

作者： Wang, Tianya 1 ; Guo, Shaomin 2 ; Jiang, Yingfen 3 ; Zou, Jun 4 ; Yu, Kunjiang 1 ; Khattak, Aimal Nawaz 1 ; Tian, Enta 1 ;

作者机构： 1.Guizhou Univ, Agr Coll, Guiyang 550025, Peoples R China

2.Guizhou Acad Agr Sci, Inst Rapeseed Res, Guiyang, Peoples R China

3.Anhui Acad Agr Sci, Inst Crop Res, Hefei, Peoples R China

4.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China

关键词： Brassica carinata; anthocyanin; iTRAQ; proteomic analysis; transcriptome analysis

期刊名称：JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY （ 影响因子：1.641； 五年影响因子：1.616 ）

ISSN： 1462-0316

年卷期： 2021 年 96 卷 4 期

页码：

收录情况： SCI

摘要： Anthocyanins are the main pigments in leaves, flowers and fruits, performing diverse biological functions in plants and exhibiting benefits for human health. The purple-leaved accession of Brassicacarinata was used to study the biosynthetic mechanism of anthocyanins in this study. To elucidate the mechanisms of anthocyanin accumulation in the purple-leaved line, we employed comparative transcriptome and iTRAQ-based proteome technique to investigate the expression profile of the purple-leaved line (BC-P01) in comparison with that of the green-leaved line (BC-G01) in B. carinata. In total, 58,124 transcripts and 4,631 proteins were identified, of which 5,183 and 285 exhibited significant changes in abundance between BC-G01 and BC-P01. Joint transcriptome and proteome analysis reveal six candidate genes of UDP-glucosyl transferase 79B1 (UGT79B1) for synthesis of cyanidin 3-O-xylosyl(1->2)glucoside, UDP-glucosyl transferase 79C1 (UGT75C1) for synthesis of cyanidin 3-O-xylosyl(1->2)glucoside, chalcone isomerase (CHI) and chalcone synthase (CHS) for synthesis of chalcone, anthocyanidin 5-O-glucoside-6-O-malonyltransferase (AT5MAT) for malonylation of anthocyanins, granule bound starch synthase 1 (GBSS1) for amylose starch biosynthetic, glutathione s-transferase f3 (GSTF3) for encoding glutathione transferase for anthocyanin transporting, respectively. The expression level of the transcriptome data was further verified by qRT-PCR method. The study can provide useful information for biosynthesis of total anthocyanin content (TAC) and molecular breeding in B. carinata and other species in Brassiceae.

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