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Proteomic changes induced by ascorbic acid treatment on porcine immature Sertoli cells

文献类型： 外文期刊

作者： Yang, Cai-Xia 1 ; Yang, Yu-Wei 2 ; Mou, Qiao 2 ; Chen, Lu 2 ; Wang, Chonglong 3 ; Du, Zhi-Qiang 1 ;

作者机构： 1.Yangtze Univ, Coll Anim Sci, Jingzhou 434025, Hubei, Peoples R China

2.Northeast Agr Univ, Coll Anim Sci & Technol, Harbin 150030, Heilongjiang, Peoples R China

3.Anhui Acad Agr Sci, Inst Anim Husb & Vet Med, Key Lab Pig Mol Quantitat Genet Anhui Acad Agr Sc, Anhui Prov Key Lab Livestock & Poultry Prod Safet, Hefei 230031, Peoples R China

关键词： Pig; Ascorbic acid; Immature sertoli cells; Proteomics

期刊名称：THERIOGENOLOGY （ 影响因子：2.923； 五年影响因子：2.843 ）

ISSN： 0093-691X

年卷期： 2022 年 188 卷

页码：

收录情况： SCI

摘要： Somatic Sertoli cells constitute the microenvironment and produce essential substances, to support male germ cell development and maturation in testis. We previously found that ascorbic acid treatment of porcine immature Sertoli cells enhances its proliferation and secretion of reproductive hormones and metabolites, and reprograms the global transcriptome. Proteomics is a powerful tool to systematically profile the underlying protein changes. Here, by employing the TMT-based quantitative proteomics method, we identified 96 and 64 significantly up-and down-regulated proteins in porcine immature Sertoli cells treated by ascorbic acid, respectively. Gene enrichment (GO and KEGG) and protein-protein interaction analyses revealed important molecular pathways (dioxygenase activity, sterol biosynthetic process, PI3K-Akt, negative regulation of peptide hormone secretion, extracellular matrix etc.). Further validation of three proteins, HMGCS1 (cholesterol synthesis), P4HA1 (glycolysis) and KDM5A (demethylation of histone 3 at lysine 4), confirmed their significant differential abundance, respectively. Taken together, our findings show that ascorbic acid can alter multiple important protein molecules and related signaling pathways, which could explain partially phenotypic changes (proliferation, apoptosis, nucleic acid methylation, lactate and reproductive hormone secretion) of porcine immature Sertoli cells as induced by ascorbic acid.

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