文献类型： 外文期刊
作者： Wang Chao-nan 1 ; Luan Fei-shi 2 ; Liu Hong-yu 2 ; Davis, Angela R. 2 ; Zhang Qi-an 3 ; Dai Zu-yun 4 ; Liu Shi 2 ;
作者机构： 1.ortheast Agr Univ, Hort & Landscape Architecture Coll, Minist Agr & Rural Affairs, Key Lab Biol & Genet Improvement Hort Crops Nort, Harbin 150030, Peoples R China
2.Agr Res Serv, US Dept Agr, South Cent Agr Res Lab, Washington, DC 94710 USA
3.Anhui Acad Agr Sci, Inst Hort, Hefei 230031, Peoples R China
4.Anhui Jianghuai Hort Technol Co Ltd, Hefei 230031, Peoples R China
关键词： watermelon; fine mapping; flesh color; QTL; transcriptome
期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）
ISSN： 2095-3119
年卷期： 2021 年 20 卷 8 期
页码：
收录情况： SCI
摘要： The color of watermelon flesh is an important trait determined by a series of carotenoids. Herein, we used Cream of Saskatchewan (pale yellow flesh) and PI 186490 (white flesh) as parental materials for an F2 segregation and initial mapping using the bulked segregant analysis sequencing (BSA-seq) strategy. The BSA results revealed a flesh colorrelated QTL that spans approximately 2.45 Mb on chromosome 6. This region was preliminarily positioned in a 382-kb segment, and then narrowed down into a 66.8-kb segment with 1 260 F2 individuals. A total of nine candidate genes were in the fine mapping interval, but only Cla007528 (encoding chlorophyllase) had non-synonymous mutations and was significantly expressed between the parental materials throughout flesh development. We also checked the expression patterns of the carotenoid metabolic pathway genes based on RNA-seq data and qRT-PCR validation. Three genes in the xanthophyll cycle (ClCHYB, ClNCED-1 and ClNCED-7) exhibited differential expression patterns between the two parental lines at different flesh color formation stages. ClPSY1, ClPDS, ClZDS, ClCHXE, ClCRTISO and ClLCYB also exhibited clearly different expression patterns accompanied by carotenoid accumulation.
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